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linear gradient elution

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  • Multicolumn countercurrent solvent gradient purification — (MCSGP) is a form of chromatography that is used to separate or purify biomolecules from complex mixtures. It was developed at the Swiss Federal Institute of Technology Zürich by Aumann and Morbidelli.[1] The process consists of three to six… …   Wikipedia

  • High-performance liquid chromatography — An HPLC. From left to right: A pumping device generating a gradient of two different solvents, a steel enforced column and an apparatus for measuring the absorbance. Acronym HPLC Classification Chromatography …   Wikipedia

  • High performance liquid chromatography — Infobox chemical analysis name = High performance liquid chromatography caption =A HPLC. From left to right: A pumping device generating a gradient of two different solvents, a steel enforced column and an apparatus for measuring the absorbance.… …   Wikipedia

  • Displacement chromatography — is a chromatography technique in which a sample is placed onto the head of the column[n 1] and is then displaced by a solute that is more strongly sorbed than the components of the original mixture. The result is that the components are resolved… …   Wikipedia

  • chromatography — chromatographer, n. chromatographic /kreuh mat euh graf ik, kroh meuh teuh /, adj. chromatographically, adv. /kroh meuh tog reuh fee/, n. Chem. the separation of mixtures into their constituents by preferential adsorption by a solid, as a column… …   Universalium

  • Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup …   Wikipedia

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

  • separation and purification — ▪ chemistry Introduction       in chemistry, separation of a substance into its components and the removal of impurities. There are a large number of important applications in fields such as medicine and manufacturing. General principles… …   Universalium

  • Column chromatography — A chemist in the 1950s using column chromatography. The Erlenmeyer receptacles are on the floor. Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for… …   Wikipedia

  • analysis — /euh nal euh sis/, n., pl. analyses / seez /. 1. the separating of any material or abstract entity into its constituent elements (opposed to synthesis). 2. this process as a method of studying the nature of something or of determining its… …   Universalium

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